Specialized Metabolites from Ribosome Engineered Strains of Streptomyces clavuligerus

Bacterial specialized metabolites are of immense importance because their medicinal, industrial, and agricultural applications. Streptomyces clavuligerus is a known producer such compounds; however, much its metabolic potential remains unknown, as many associated biosynthetic gene clusters silent or expressed at low levels. The overexpression ribosome recycling factor (frr) engineering (induced rpsL mutations) in other spp. has been reported to increase the production metabolites. Therefore, we used an strategy combination with untargeted metabolomics, molecular networking, silico analysis annotate 28 current study, which have not previously S. clavuligerus. Many newly described commonly found plants, further alluding ability produce compounds under specific conditions. In addition, manipulation frr led different metabolite profiles most cases. Known putative observed also discussed. This work suggests that traditional strain recently developed metabolomics technologies together can provide rapid cost-effective strategies speed up discovery novel natural products.